Lab Equipment

Two inverted microscopes from Nikon (Ti2 Eclipse) illuminated at four different wavelengths (405, 488, 561, and 635 nm) by high powered lasers (>100 mW) housed in laser combiners (from Hubner and Oxxius respectively). The combiners are connected to the microscope by optical fibers to TIRF-E arms (Nikon), which controls laser angles for TIRF imaging. Both microscopes use Prime95B sCMOS cameras (Teledyne Photometrics) allowing fast data acquisition over a large field of view, and are fitted with stage top incubators for long-term live cell imaging. One microscope is also fitted with a OptoSplit II system (Cairn Research) for simultaneous imaging of two different fluorophores.